Advantages of tissue microtome

There are many types of microtome, and the scope of application of each type of slicer is different. The tissue microtome has many advantages when it is used. As long as you use the microtome according to the prescribed method, you can ensure that it has a good use effect. The editor of the microtome manufacturer will introduce the advantages of the microtome to you.

Tissue microtome is a kind of equipment for pathological section analysis of human tissues, animal and plant tissues. Tissue microtome are generally divided into rotary microtome and frozen microtome.

Rotary microtome: It is used to slice by rotating the hand wheel. The wax block table is embedded in the metal clamp seat that can move up and down in the groove, and cuts the flat slice by pushing forward with the micro screw. Some rotary microtome are equipped with three knobs and a fastening knob on the head, which can deflect and tighten it in all directions, so as to adjust the section of the wax block. The cutting angle of the slicer can be adjusted. Because this microtome uses a heavy and large slicer knife, it generally does not vibrate when cutting hard tissue. The slice thickness can be adjusted from 1-30 microns to any thickness by the knob, and each gradient is 1 or 2 microns.

The advantage of the rotary microtome is that the body is heavy, so it is more stable than the rocking microtome. It is very suitable for cutting paraffin sections. It can be ideal for cutting continuous sections and also for cutting large tissue blocks.

Frozen sectioning is a technical method that uses physical cooling to freeze fresh tissue samples to produce certain hardness for sectioning. Because there is no need for dehydration and embedding, the production speed is faster than paraffin section, which is a good method for clinicians to provide pathological diagnosis during surgery. In addition, frozen section is also an ideal preparation method for fat staining, enzyme histochemical staining, some immunohistochemical staining and in situ hybridization because the sample of frozen section is fresh and unfixed tissue. The disadvantage of frozen section is that the morphology of tissue cells is slightly inferior to that of paraffin section.

Frozen microtome are widely used in hospitals, medical schools, forensic medicine, life sciences, health and epidemic prevention, public security forensic medicine, agricultural colleges, scientific research institutions and other institutions to make pathological and biological tissue sections, and animal and plant scientific research institutions to make pathological diagnosis, analysis and research.